Somatic embryogenesis from vegetative shoot apices of mature trees of Pinus patula
نویسندگان
چکیده
منابع مشابه
The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees.
Investigations were undertaken on the use of somatic embryogenesis to generate cocoa swollen shoot virus (CSSV) disease free clonal propagules from infected trees. Polymerase chain reaction (PCR) capillary electrophoresis revealed the presence of CSSV in all the callus tissues induced from the CSSV-infected Amelonado cocoa trees (T1, T2 and T4). The virus was transmitted to primary somatic embr...
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Within a plantation of clonal somatic embryo-derived white spruce trees that belonged to four genotypes, one genotype (G6) has consistently responded for the last 16 years, to the induction of somatic embryogenesis within primordial shoot explants. Analysis of fourteen individuals within this genotype subsequently revealed a group of clonal trees that were nonresponsive. This in turn provided a...
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The focus of the current project was to establish somatic embryogenesis protocols for the tropical pine species Pinus oocarpa using immature zygotic embryos (ZEs) as explants. Somatic embryogenesis is best supported by mimicking natural seed-embryo developmental conditions, through a tissue culture medium formulation based on the mineral content of the seed nutritive tissue [megagametophyte (MG...
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An anaerobic, mesophilic, sporeforming, xylanolytic bacterium was isolated from decayed Pinus patula wood chips. The cells of this organism were gram-negative rods, had peritrichous flagella, and formed terminal round endospores which distended the cell wall. Xylan, cellobiose, fructose, glucose, maltose, mannose, melezitose, raffinose, rhamnose, salicin, sucrose, and xylose were utilized. The ...
متن کاملSomatic Embryogenesis and Massive Shoot Regeneration from Immature Embryo Explants of Tef
Tef (Eragrostis tef) provides a major source of human nutrition in the Horn of Africa, but biotechnology has had little impact on its improvement to date. Here, we report the elaboration of an in vitro regeneration protocol, based on the use of immature zygotic embryos as explant. Explant size was an important determinant of in vitro regeneration efficiency, as was the formulation of the cultur...
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ژورنال
عنوان ژورنال: Tree Physiology
سال: 2005
ISSN: 0829-318X,1758-4469
DOI: 10.1093/treephys/25.1.11